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- SMAD4 Antibodies
Invitrogen
View all (68) SMAD4 antibodies
Cite SMAD4 Recombinant Rabbit Monoclonal Antibody (23GB975)
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SMAD4 Antibody (MA5-54080) in ICC/IF
Immunocytochemistry analysis of SMAD4 in C2C12 cells. Nuclei were stained blue with DAPI; SMAD4 recombinant monoclonal antibody (Product # MA5-54080) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
MA5-54080
Applications
Tested Dilution
Publications
Western Blot(WB)
1:1,000-1:5,000
-
Immunocytochemistry(ICC/IF)
1:1,000
-
Flow Cytometry(Flow)
1:2,000
-
Product Specifications
Species Reactivity
Human,Mouse,Rat
Host/Isotype
Rabbit/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB975
Immunogen
A synthetic peptide of human Smad4 (500-552AA).
Conjugate
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; hSMAD4; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4
UniProt ID:(Human) Q13485, (Mouse) P97471, (Rat) O70437
Entrez Gene ID:(Human) 4089, (Mouse) 17128, (Rat) 50554
Molecular Function: 
DNA-binding transcription factor gene-specific transcriptional regulator
Function(s)
negative regulation of transcription from RNA polymerase II promoterovarian follicle developmentbranching involved in ureteric bud morphogenesisresponse to hypoxiain utero embryonic developmentgastrulation with mouth forming secondatrioventricular valve formationepithelial to mesenchymal transition involved in endocardial cushion formationleft ventricular cardiac muscle tissue morphogenesispositive regulation of cell proliferation involved in heart valve morphogenesisbrainstem developmenttranscription from RNA polymerase II promotercellular iron ion homeostasistransforming growth factor beta receptor signaling pathwaySMAD protein complex assemblyspermatogenesissingle fertilizationaxon guidanceendoderm developmentmesoderm developmentcell proliferationnegative regulation of cell proliferationnegative regulation of cardiac muscle hypertrophypositive regulation of epithelial to mesenchymal transitionpositive regulation of pathway-restricted SMAD protein phosphorylationneural crest cell differentiationregulation of transforming growth factor beta receptor signaling pathwaynegative regulation of cell growthBMP signaling pathwaypositive regulation of transforming growth factor beta receptor signaling pathwaypositive regulation of BMP signaling pathwaysomite rostral/caudal axis specificationregulation of transforming growth factor beta2 productionpositive regulation of luteinizing hormone secretionsomatic stem cell population maintenanceintracellular signal transductionatrioventricular canal developmentendothelial cell activationembryonic digit morphogenesisnegative regulation of transcription, DNA-templatedpositive regulation of transcription, DNA-templatedpositive regulation of transcription from RNA polymerase II promoterpositive regulation of follicle-stimulating hormone secretiondevelopmental growthneuron fate commitmentsebaceous gland developmentformation of anatomical boundaryregulation of bindingpositive regulation of histone H3-K4 methylationregulation of hair follicle developmentpalate developmentuterus developmentpositive regulation of SMAD protein import into nucleusSMAD protein signal transductionventricular septum morphogenesisnegative regulation of cell deathendocardial cell differentiationfemale gonad morphogenesisinterleukin-6-mediated signaling pathwaynegative regulation of ERK1 and ERK2 cascaderesponse to transforming growth factor betacellular response to BMP stimulusmetanephric mesenchyme morphogenesisnephrogenic mesenchyme morphogenesisseminiferous tubule developmentpositive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimuluspositive regulation of histone H3-K9 acetylationkidney developmentcardiac septum developmenttranscription, DNA-templatedregulation of transcription, DNA-templatedregulation of transcription from RNA polymerase II promotergastrulationanterior/posterior pattern specificationregulation of cell proliferationnegative regulation of protein catabolic processtissue morphogenesis
Process(es)
RNA polymerase II core promoter proximal region sequence-specific DNA bindingcore promoter proximal region sequence-specific DNA bindingtranscription factor activity, protein bindingtranscription factor activity, RNA polymerase II transcription factor bindingtranscriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific bindingRNA polymerase II transcription factor bindingDNA bindingchromatin bindingtranscription factor activity, sequence-specific DNA bindingprotein bindingcollagen bindingtransforming growth factor beta receptor, common-partner cytoplasmic mediator activityidentical protein bindingprotein homodimerization activitysequence-specific DNA bindingtranscription regulatory region DNA bindingmetal ion bindingprotein heterodimerization activityI-SMAD bindingR-SMAD bindingRNA polymerase II regulatory region sequence-specific DNA bindingtranscriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific bindingfilamin bindingSMAD binding
It has to be done as per old AB suggested Products section.
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